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Alternative Splicing
 
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Biology Professor (Twitter: @DrWhitneyHolden) teaches a lesson about alternative splicing, the highly regulated process in eukaryotic cells that allows one gene to code for multiple proteins, with a focus on the diversity this brings to the genome and on the mechanism of alternative splicing, including the spliceosome
Views: 21918 Biology Professor
Alternative Splicing / Alternative Processing (Eukaryotes)
 
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Moof's Medical Biochemistry Video Course: http://moof-university.thinkific.com/courses/medical-biochemistry-for-usmle-step-1-exam
Views: 75323 Moof University
Alternative splicing
 
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Alternative splicing - This lecture explains about the alternative splicing process to generate different varieties of mRNA from a single gene. Alternative splicing is a regulated approach for the duration of gene expression that results in a single gene coding for more than one proteins. In this approach, designated exons of a gene could also be included inside or excluded from the final, processed messenger RNA (mRNA) made from that gene.Accordingly the proteins translated from then again spliced mRNAs will include variations in their amino acid sequence and, most likely, of their biological services (see figure). Mainly, alternative splicing makes it possible for the human genome to direct the synthesis of many extra proteins than would be anticipated from its 20,000 protein-coding genes. Replacement splicing is mostly termed differential splicing. Watch this video lecture to understand the mechanism of alternative splicing. For more information, log on to- http://www.shomusbiology.com/ Get Shomu's Biology DVD set here- http://www.shomusbiology.com/dvd-store/ Download the study materials here- http://shomusbiology.com/bio-materials.html Remember Shomu’s Biology is created to spread the knowledge of life science and biology by sharing all this free biology lectures video and animation presented by Suman Bhattacharjee in YouTube. All these tutorials are brought to you for free. Please subscribe to our channel so that we can grow together. You can check for any of the following services from Shomu’s Biology- Buy Shomu’s Biology lecture DVD set- www.shomusbiology.com/dvd-store Shomu’s Biology assignment services – www.shomusbiology.com/assignment -help Join Online coaching for CSIR NET exam – www.shomusbiology.com/net-coaching We are social. Find us on different sites here- Our Website – www.shomusbiology.com Facebook page- https://www.facebook.com/ShomusBiology/ Twitter - https://twitter.com/shomusbiology SlideShare- www.slideshare.net/shomusbiology Google plus- https://plus.google.com/113648584982732129198 LinkedIn - https://www.linkedin.com/in/suman-bhattacharjee-2a051661 Youtube- https://www.youtube.com/user/TheFunsuman Thank you for watching the alternative splicing mechanism in details.
Views: 43476 Shomu's Biology
The regulation of spliceosome-mediated splicing
 
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In this animation, we explore the splicing of mRNA by the spliceosome is regulated. http://ukcatalogue.oup.com/product/9780199658572.do
Post-transcriptional regulation | Biomolecules | MCAT | Khan Academy
 
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Created by Tracy Kim Kovach. Watch the next lesson: https://www.khanacademy.org/test-prep/mcat/biomolecules/gene-control/v/non-coding-rna-ncrna?utm_source=YT&utm_medium=Desc&utm_campaign=mcat Missed the previous lesson? https://www.khanacademy.org/test-prep/mcat/biomolecules/gene-control/v/regulation-of-transcription?utm_source=YT&utm_medium=Desc&utm_campaign=mcat MCAT on Khan Academy: Go ahead and practice some passage-based questions! About Khan Academy: Khan Academy offers practice exercises, instructional videos, and a personalized learning dashboard that empower learners to study at their own pace in and outside of the classroom. We tackle math, science, computer programming, history, art history, economics, and more. Our math missions guide learners from kindergarten to calculus using state-of-the-art, adaptive technology that identifies strengths and learning gaps. We've also partnered with institutions like NASA, The Museum of Modern Art, The California Academy of Sciences, and MIT to offer specialized content. For free. For everyone. Forever. #YouCanLearnAnything Subscribe to Khan Academy’s MCAT channel: https://www.youtube.com/channel/UCDkK5wqSuwDlJ3_nl3rgdiQ?sub_confirmation=1 Subscribe to Khan Academy: https://www.youtube.com/subscription_center?add_user=khanacademy
Views: 138627 khanacademymedicine
WHAT IS ALTERNATIVE SPLICING, ALTERNATIVE SPLICING PROCESS
 
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what is alternative splicing , how alternative splicing comes, introns and exons definition, role of this in gene regulation.
Views: 403 omar danso
WHAT IS ALTERNATIVE SPLICING , THE ROLE OF ALTERNATIVE SPLICING IN GENE REGULATIONS
 
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what is an alternative splicing process and what importance does it have on gene regulation and to an organism .
Views: 117 omar danso
Alternative splicing - Melissa Moore (U Mass/HHMI)
 
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https://www.ibiology.org/genetics-and-gene-regulation/alternative-splicing/ Overview of the different types of alternative splicing and their proteomic consequences.
Views: 14108 iBiology Techniques
mRNA splicing
 
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In this animation, we explore how the splicing of mRNAs to remove introns occurs. http://ukcatalogue.oup.com/product/9780199658572.do
Drosophila Sex Determination - Including mRNA Splicing Explanation
 
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The Genetics and Biochemistry behind Sex Differentiation in Drosophila (Fruit Flies)
Views: 16906 Neural Academy
mRNA Splicing Mechanisms
 
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This video is a project I completed for my molecular genetics class. Please feel free to comment if there are any mistakes and I will promptly fix them.
Views: 100380 LunaShadowfaux
What is ALTERNATIVE SPLICING? What does ALTERNATIVE SPLICING mean? ALTERNATIVE SPLICING meaning
 
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What is ALTERNATIVE SPLICING? What does ALTERNATIVE SPLICING mean? ALTERNATIVE SPLICING meaning - ALTERNATIVE SPLICING definition - ALTERNATIVE SPLICING explanation. Source: Wikipedia.org article, adapted under https://creativecommons.org/licenses/by-sa/3.0/ license. Alternative splicing is a regulated process during gene expression that results in a single gene coding for multiple proteins. In this process, particular exons of a gene may be included within or excluded from the final, processed messenger RNA (mRNA) produced from that gene. Consequently, the proteins translated from alternatively spliced mRNAs will contain differences in their amino acid sequence and, often, in their biological functions (see Figure). Notably, alternative splicing allows the human genome to direct the synthesis of many more proteins than would be expected from its 20,000 protein-coding genes. Alternative splicing is sometimes termed differential splicing. Alternative splicing occurs as a normal phenomenon in eukaryotes, where it greatly increases the biodiversity of proteins that can be encoded by the genome; in humans, ~95% of multi-exonic genes are alternatively spliced. There are numerous modes of alternative splicing observed, of which the most common is exon skipping. In this mode, a particular exon may be included in mRNAs under some conditions or in particular tissues, and omitted from the mRNA in others. The production of alternatively spliced mRNAs is regulated by a system of trans-acting proteins that bind to cis-acting sites on the primary transcript itself. Such proteins include splicing activators that promote the usage of a particular splice site, and splicing repressors that reduce the usage of a particular site. Mechanisms of alternative splicing are highly variable, and new examples are constantly being found, particularly through the use of high-throughput techniques. Researchers hope to fully elucidate the regulatory systems involved in splicing, so that alternative splicing products from a given gene under particular conditions could be predicted by a "splicing code". Abnormal variations in splicing are also implicated in disease; a large proportion of human genetic disorders result from splicing variants. Abnormal splicing variants are also thought to contribute to the development of cancer, and splicing factor genes are frequently mutated in different types of cancer.
Views: 6312 The Audiopedia
mRNA Splicing
 
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NDSU Virtual Cell Animations Project animation 'mRNA Splicing'. For more information please see http://vcell.ndsu.edu/animations Before being used in translation, mRNA must be spliced. During splicing, introns are removed and the translatable exons that remain are spliced into a single strand of mRNA.
Views: 891517 ndsuvirtualcell
Yoseph Barash: Visualization of Alternative Splicing
 
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Copyright Broad Institute, 2013. All rights reserved. Yoseph Barash (bit.ly/jbarash) introduces how genes are alternative spliced, highlights limitations in current methods for visualizing splicing data, and speculates on how future methods may evolve. He presents his remarkable 'splicing code', recently published in Nature, which concisely explains tissue-specific splicing regulation. This talk was presented at VIZBI 2011, an international conference series on visualizing biological data (vizbi.org) funded by NIH & EMBO. Slides from the talk are at bit.ly/q0KY56. For information about data visualization efforts at the Broad Institute, please visit: http://www.broadinstitute.org/node/1363/
Views: 955 Broad Institute
S. Ralph - Regulation of alternative splicing in human parasites
 
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Stuart. A. Ralph , Department of Biochemistry & Molecular Biology, Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Victoria, AUSTRALIA speaks on "Regulation of alternative splicing in human parasites". This seminar has been recorded by ICGEB Trieste
Views: 74 Icgeb
Melissa Moore (U. Mass/HHMI) Part 1: Split Genes and RNA Splicing
 
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https://www.ibiology.org/genetics-and-gene-regulation/alternative-splicing/ In the first part of her talk, Dr. Moore explains that eukaryotic pre-mRNA contains long stretches of non-protein coding sequences interspersed with protein coding regions. By recognizing specific sequences, cellular machinery splices out the non-coding introns leaving just the protein-coding exons in mRNA. Although at first glance this may seem like a wasteful process, it is splicing that facilitates the evolution of new genes, and alternative splicing that allows a limited number of genes to produce a large number of proteins.
Views: 22851 iBiology
Genetic variants and associated proteins in the regulation of pre mRNA splicing - Grace Xiao
 
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June 10, 2016 - ENCODE 2016: Research Applications and Users Meeting More: https://www.genome.gov/27566810
Mechanism of intron removal and exon splicing
 
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Introns are removed from eukaryotic RNA transcripts and exons are spliced together. This video shows the details of the process of removal of a typical nuclear intron, including the roles of the various SNRNPs. Includes the formation of the A, B, and C complexes, and the two transesterification reactions. CC licensed images can be downloaded from my Flickr site in the "teaching" set. http://www.flickr.com/photos/agathman/
Views: 63156 Allen Gathman
Splice sites - Melissa Moore (U Mass/HHMI)
 
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https://www.ibiology.org/genetics-and-gene-regulation/alternative-splicing/ How do cells know where to splice the RNA? How conserved is this process?
Views: 1912 iBiology Techniques
Alternative Splicing - Molecular Biology  (Lecture 131)
 
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Alternative Splicing Molecular Biology
Splicing mutations - Melissa Moore (U Mass/HHMI)
 
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https://www.ibiology.org/genetics-and-gene-regulation/alternative-splicing/ Mutations that cause changes in splicing patterns are frequent causes of human diseases.
Views: 7037 iBiology Techniques
ALTERNATE SPLICING OF INTRONS
 
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Description
Views: 4459 Walter Jahn
Splicing Mechanism - Melissa Moore (U Mass/HHMI)
 
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Dr. Moore describes the mechanism of splicing, including how splice sites are chosen, exon definition, the chemical steps of splicing, and the spliceosome cycle.
Views: 32196 iBiology Techniques
RNA splicing part 1 (introduction)
 
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For more information, log on to- http://shomusbiology.weebly.com/ Download the study materials here- http://shomusbiology.weebly.com/bio-materials.html Source of the article published in description is Wikipedia. I am sharing their material. Copyright by original content developers of Wikipedia. Link- http://en.wikipedia.org/wiki/Main_Page
Views: 89855 Shomu's Biology
RNA Processing in eukaryotes | RNA splicing, capping and editing
 
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RNA processing lecture - This lecture explains about the different RNA processing stages and mechanism of RNA processing in eukaryotes including the RNA splicing and processing mechanisms like RNA splicing, RNA capping, polyadenylation and RNA editing. Here you will learn the RNA splicing and processing mechanism and the role of RNA processing in eukaryotes to make the eukaryotic mature RNA. This mature eukaryotic RNA can be translated into protein products in cytosol. For more information, log on to- http://www.shomusbiology.com/ Get Shomu's Biology DVD set here- http://www.shomusbiology.com/dvd-store/ Download the study materials here- http://shomusbiology.com/bio-materials.html Remember Shomu’s Biology is created to spread the knowledge of life science and biology by sharing all this free biology lectures video and animation presented by Suman Bhattacharjee in YouTube. All these tutorials are brought to you for free. Please subscribe to our channel so that we can grow together. You can check for any of the following services from Shomu’s Biology- Buy Shomu’s Biology lecture DVD set- www.shomusbiology.com/dvd-store Shomu’s Biology assignment services – www.shomusbiology.com/assignment -help Join Online coaching for CSIR NET exam – www.shomusbiology.com/net-coaching We are social. Find us on different sites here- Our Website – www.shomusbiology.com Facebook page- https://www.facebook.com/ShomusBiology/ Twitter - https://twitter.com/shomusbiology SlideShare- www.slideshare.net/shomusbiology Google plus- https://plus.google.com/113648584982732129198 LinkedIn - https://www.linkedin.com/in/suman-bhattacharjee-2a051661 Youtube- https://www.youtube.com/user/TheFunsuman Thank you for watching RNA processing in eukaryotes lecture
Views: 105132 Shomu's Biology
RNA Splicing
 
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A 3D animation shows the crucial RNA editing step called splicing Originally created for Learn About Spinal Muscular Atrophy ( http://www.learnaboutsma.org/ ) TRANSCRIPT: As DNA is transcribed into RNA it needs to be edited to remove non-coding regions, or introns, shown in green. This editing process is called splicing, which involves removing the introns, leaving only the yellow, protein-coding regions, called exons. RNA splicing begins with assembly of helper proteins at the intron/exon borders. These splicing factors act as beacons to guide small nuclear ribo proteins to form a splicing machine, called the spliceosome. The animation is showing this happening in real time. The spliceosome then brings the exons on either side of the intron very close together, ready to be cut. One end of the intron is cut and folded back on itself to join and form a loop. The spliceosome then cuts the RNA to release the loop and join the two exons together. The edited RNA and intron are released and the spliceosome disassembles. This process is repeated for every intron in the RNA. Numerous spliceosomes, shown here in purple, assemble along the RNA. Each spliceosome removes one intron, releasing the loop before disassembling. In this example, three introns are removed from the RNA to leave the complete instructions for a protein.
Views: 204325 DNA Learning Center
RNA Splicing [HD Animation]
 
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RNA Splicing [HD Animation]
Views: 56120 Health Care
Intron retention and alternative splicing ...
 
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Alternative splicing and intron retention Want to learn more about how we are investigating this phenomenon in cancer? Link below will take you to a lay friendly description; https://lantsandlaminins.wordpress.com/about/taking-control-of-a-cancer-associated-splicing-switch/
Views: 313 Kevin Hamill
Alternative splicing - Medical Definition
 
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https://word2speech.com/medical/ Alternative splicing Alternative splicing: A mechanism by which different forms of mature mRNAs (messengers RNAs) are generated from the same gene. Alternative splicing is a regulatory mechanism by which variations in the incorporation of the exons, or coding regions, into mRNA leads to the production of more than one related protein, or isoform. How to pronounce, definition of, audio dictionary, medical dictionary
Views: 92 Medical Dictionary
Post-Transcriptional Modifications of mRNA
 
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Donate here: http://www.aklectures.com/donate.php Website video link: http://www.aklectures.com/lecture/post-transcriptional-modifications-of-mrna Facebook link: https://www.facebook.com/aklectures Website link: http://www.aklectures.com
Views: 87236 AK LECTURES
RNA Splicing Animation | self splicing
 
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In molecular biology, RNA splicing is the editing of the nascent pre-messenger RNA (pre-mRNA) transcript. After splicing, introns are removed and exons are joined together (ligated). For nuclear-encoded genes, splicing takes place within the nucleus either co-transcriptionally or immediately after transcription. For those eukaryotic genes that contain introns, splicing is usually required in order to create an mRNA molecule that can be translated into protein. For many eukaryotic introns, splicing is carried out in a series of reactions which are catalyzed by the spliceosome, a complex of small nuclear ribonucleoproteins (snRNPs). Self-splicing introns, or ribozymes capable of catalyzing their own excision from their parent RNA molecule, also exist. Self-splicing occurs for rare introns that form a ribozyme, performing the functions of the spliceosome by RNA alone. There are three kinds of self-splicing introns, Group I, Group II and Group III. Group I and II introns perform splicing similar to the spliceosome without requiring any protein. This similarity suggests that Group I and II introns may be evolutionarily related to the spliceosome. Self-splicing may also be very ancient, and may have existed in an RNA world present before protein. Two transesterifications characterize the mechanism in which group I introns are spliced: 3'OH of a free guanine nucleoside (or one located in the intron) or a nucleotide cofactor (GMP, GDP, GTP) attacks phosphate at the 5' splice site. 3'OH of the 5' exon becomes a nucleophile and the second transesterification results in the joining of the two exons. The mechanism in which group II introns are spliced (two transesterification reaction like group I introns) is as follows: The 3'OH of a specific adenosine in the intron attacks the 5' splice site, thereby forming the lariat The 3'OH of the 5' exon triggers the second transesterification at the 3' splice site, thereby joining the exons together. Thank You for watching our videos from Biology Animation Videos channel. This channel is created to compile animated biology lectures and videos from different animation sources. None of these videos are created by us. we just organize them and place them in YouTube for your understanding so If you want to know details about these animation please see the credit section for knowing the original content developer and please convey privilege and gratitude to them. Thank You. Our website- https://www.biologyanimationvideos.weebly.com Thank you for watching RNA splicing animation.
BAD tutorial example - Alternative splicing of mRNA explained
 
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This is an example of a bad biology tutorial that I created to show our Honors students what NOT to do when making their own tutorials. This has far too much information and is not geared appropriately toward their audience. In it, someone tries to tell someone else about mRNA, with mixed results.
Exons and Introns of Eukaryotic mRNA
 
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Donate: here: http://www.aklectures.com/donate.php Website video link: http://www.aklectures.com/lecture/exons-and-introns-of-eukaryotic-mrna Facebook link: https://www.facebook.com/aklectures Website link: http://www.aklectures.com
Views: 49667 AK LECTURES
VIALS - VIsualizing ALternative Splicing in Genes
 
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more info: http://vials.io Alternative splicing is a process by which the same DNA sequence is used to assemble different proteins, called protein isoforms. Alternative splicing works by selectively omitting some of the coding regions (exons) typically associated with a gene. Detection of alternative splicing is difficult and uses a combination of advanced data acquisition methods and statistical inference. Knowledge about the abundance of isoforms is important for understanding both normal processes and diseases and to eventually improve treatment through targeted therapies. The data, however, is complex and current visualizations for isoforms are neither perceptually efficient nor scalable. To remedy this, we developed Vials, a novel visual analysis tool that enables analysts to explore the various datasets that scientists use to make judgments about isoforms: the abundance of reads associated with the coding regions of the gene, evidence for junctions, i.e., edges connecting the coding regions, and predictions of isoform frequencies.
Views: 1364 Hendrik Strobelt
Alternative RNA Splicing
 
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Researchers Dr. Phil Sharp and Dr. Adrian Krainer describe alternative splicing of genes Originally created for Learn About Spinal Muscular Atrophy ( http://www.learnaboutsma.org/ ) TRANSCRIPT: Phil Sharp: Many genes in our body are expressed as RNA in different cell types, a skin cell, a bone cell, a brain cell. But in one cell type, the pattern of splicing of the sense pieces together is different than the pattern in another cell type and that's called alternative splicing. So it's alternative splicing because the splicing pattern in one cell is different than the splicing pattern in another cell and therefore the proteins in those two cells are different. And that can make a great deal of difference because one protein can function, splice in one pattern in one cell one way and then another spliced differently in another way. So, you know, if you're designing drugs or you're looking at functionality of mutations they can differ due to this alternative splicing between different cells. Adrian Krainer: Alternative splicing means that the splicing machinery selects certain exons or parts of certain exons differently in a proportion of the molecules that it splices. So it's a way to take a gene and from its primary transcript, generate two or more mRNAs that can encode two or more proteins. The splicing machinery has a choice. It sees this string of exons and it chooses what to join to what. It isn't always product A or product B, you could have some proportion of the molecules choose pathway A and the rest choose pathway B and that is the case with SMN2. If it were to completely skip exon 7, that maybe a lethal change in the absences of SMN1. In reality, it makes some full length protein.
Views: 8112 DNA Learning Center
RNA splicing
 
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Very basic video to describe RNA splicing and then some information on how mRNA can be purified. Not meant to be graduate level information, just an introduction.
Views: 112086 Greg Petersen
Splicing
 
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00:00 Review of 5' RNA capping and 3' poly-A tailing 00:55 How splicing was discovered; exons and introns 03:05 Splicing occurs co-transcriptionally 03:36 Splicing fidelity and patterns 04:35 Exon skipping 05:31 Intron retention 06:00 Mutually exclusive exons 06:35 Regulation of splicing (intron removal) 09:05 Common splice pattern 09:31 Alternative splicing produces diversity 09:45 Question for class
Views: 814 Joseph Ross
RNA processing: 5' cap, 3' polyA tail, and Splicing
 
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PCB2131, University of West Florida, Dr. Cavnar In this video we'll review the three main events involved in turning pre-mRNA into mRNA. From Topic 14.
Views: 124577 Peter Cavnar
Alternative splicing regulation in cancer initiation and progression.
 
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My Another Channel- https://goo.gl/jYW7bK https://goo.gl/rmHv17 || असल 24 न्यूज़ || सच्चाई आप तक || About Channel :- असल 24 न्यूज़ भारत का सर्वश्रेष्ठ हिंदी न्यू ज चैनल है । असल 24 न्यूज चैनल राजनीति, मनोरंजन, बॉलीवुड, व्यापार और खेल में नवीनतम समाचारों को शामिल करता है। "FOLLOW ASAL 24 NEWS " Facebook - https://www.facebook.com/asal24news/ Instagram - https://www.instagram.com/asal24news/ Twitter - https://twitter.com/asal24news Subscribe network channels: https://goo.gl/7mxPL3 Follow us on Twitter https://twitter.com/asal24news Follow us on instagram https://www.instagram.com/asal24news/ Follow us on youtube https://goo.gl/7mxPL3 Follow us on Facebook Page: www.facebook.com/asal24news/ https://www.facebook.com/asal24news/
Views: 54 Asal 24 News
Alternative Splicing Animation
 
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Mohamad Izzat Bin Mohamad - A15MB0220 Muhammad Amirul Bin Abdul Razak - A15MB0070
Views: 38 Izzat Mohamad
alternative splicing
 
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Views: 117 faiqah amzah
mRNA Splicing
 
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In most eukaryotic genes, coding regions (exons) are interrupted by noncoding regions (introns). During transcription, the entire gene is copied into a pre-mRNA, which includes exons and introns. During the process of RNA splicing, introns are removed and exons joined to form a contiguous coding sequence. This mature mRNA is ready for translation.
Views: 4952 William Orfanos
Expression Analysis of Tumors Based on Patterns in Alternative Splicing
 
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Elizabeth Purdom, UC Berkeley Computational Cancer Biology https://simons.berkeley.edu/talks/elizabeth-purdom-02-05-2016
Views: 326 Simons Institute
An Explanation of RNA splicing
 
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Nobel Laureate Dr. Phil Sharp explains the process of RNA splicing Originally created for Learn About Spinal Muscular Atrophy ( http://www.learnaboutsma.org/ ) TRANSCRIPT: Oh, well, the discovery I made in the '77 was of the split gene nature of the genes in human cells. What I mean by that is that if you look at the information within a gene and in terms of the DNA sequence, it is interrupted by non-informational DNA or nonsense DNA. The nonsense DNA is called introns, the sense DNA is called exons. So you get exon, intron, exon, intron type patterns in genes. They have to remove the nonsense for the gene work, the cell does, and it does that by splicing out the nonsense and putting the sense segments together. The cell in essence edits the structure of a gene to make the information that is then used by the cell as a functional unit for cellular activities. So we discovered that in 1977 in Cold Spring Harbor in parallel and independently made a similar discovery.
Views: 6493 DNA Learning Center